Summary of Paramyxovirinae matrix protein nuclear trafficking phenotypes.

(-),+/-, 10–25%;+, 25–50%;++, 50–75%;+++, 75–100%;++++,>100%;IP-IB, immunoprecipitation followed by immunoblot;MIP, maximum intensity projection of 3D confocal micrographs;BiFC, bimolecular fluorescence complementation; XYZ, XYZ planes view of 3D confocal micrographs;N.D., not determined;*, NES mutation gave unexpected increased nuclear exclusion rather than the expected increased nuclear localization (see Fig. 2H for quantification);**, Subcellular localization phenotypes of the NES and the various NLSbp2 mutants for NDV-M and MeV-M are not congruent (compare S3 Fig. and Fig. 2F and 2G);***, Recombinant MuV rescued with NES or NLSbp2 mutations. Defects in viral morphogenesis were observed but subcellular localization of MuV-M could not be determined due to a lack of specific anti-MuV-M antibodies.Summary of Paramyxovirinae matrix protein nuclear trafficking phenotypes.