Microfluidic device with brain extracellular matrix promotes structural and functional maturation of human brain organoids. Microfluidic device with brain extracellular matrix promotes structural and functional maturation of human brain organoids

We established a platform for the brain organoid culture by using human decellularized brain extracellular matrix (BEM) and a microfluidic device. This engineering concept of reconstituting brain-mimetic microenvironments facilitates development of a reliable culture platform for brain organoids, enabling effective modeling and drug development. Overall design: Matrigel-encapsulated brain organoid vs BEM-encapsulated brain organoid and conventional plate culture encapsulated with BEM vs microfluidic culture encapsulated with BEM.