METHOD FOR SITE-SPECIFIC MUTAGENESIS OF MEDICAGO SATIVA GENE BY USING CRISPR/CAS9 SYSTEM
收藏国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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A method for site-specific mutagenesis of a medicago sativa gene by using a CRISPR/Cas9 system. The method comprises: first constructing a universal binary expression vector MsCRISPR/Cas9 mediated and transformed by agrobacterium tumefaciens in medicago sativa; then designing a CRISPR/Cas9-based sgRNA sequence for a target gene in the medicago sativa， and connecting a DNA fragment for encoding the sgRNA sequence to MsCRISPR/Cas9 to construct a carrier MsCRISPR/Cas9::target; and then mediating and transforming the medicago sativa by using the agrobacterium tumefaciens， and obtaining， by screening， a mutant transformed plant with the target gene knocked out. According to the method， an MtU6 promoter is used for initiating sgRNA transcription in the medicago sativa.
提供机构：
国家林业和草原科学数据中心
创建时间：
2023-02-12
