Lentivector information
收藏DataCite Commons2020-08-29 更新2024-07-27 收录
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https://figshare.com/articles/Lentivector_information/6489431/1
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The lentivector Psico was purchased from Addgene (#11578, Addgene, MA, USA). After being double digested with HpaI /XhoI, the vector was connected with synthetic SP1 knockdown or NC sequences. After screened through sequencing, the positive lentivectors carrying SP1 knock-down sequence or NC were selected for cell infection. PCI-37B cells cultivated in 6-well plate at a confluence of 70% were cocultured with lentivirus suspension (SP1 knock-down 1:100; NC 1:200) for 48 hours. Then cells were cultured with complete culture medium with 15ug/ml puromycin for one week to harvest strains that have stably low levels of SP1 expression.
提供机构：
figshare
创建时间：
2019-02-08
