Integrated microRNA and secretome analysis of human endometrial organoid reveal the miR-3194-5p/Aquaporin/S100A9 module in regulating trophoblast functions

We adopted the endometrial organoid model to simulate the hormonal conditions in the menstrual cycle and early gestation. We then established the first secretome profiles and miRNA atlas of these endometrial organoid in relation to their hormonal changes. We demonstrated for the first time that the miR-3194-5p/S100A9 axis is a key regulator of trophoblast invasion during early pregnancy. Overall design: Study the effect of organoid secretion in proliferative phase, secretary phase and early pregnancy phase conditions on the functions on EVCTs in vitro.