The Identification and Functional Analysis of CircRNAs in Endometrial Receptivity of Mice with Polycystic Ovary Syndrome

The disorders of endometrial receptivity and ovulatory dysfunction are both important causes of infertility of patients with Polycystic Ovary Syndrome (PCOS). The study aimed to investigate the expression profile and functional analysis of circRNAs in the endometrial receptivity of mice with PCOS. Twenty-four 4-week-old female C57BL/6 mice were randomly divided into two groups (PCOS group and Normal control group, n=12) and PCOS group was subcutaneously with DHEA 6mg/kg for 20 days. The circRNA expression profile in the endometrium tissue of two groups on the 4th day of gestation was screened by gene chip technique. In this experiment, 24 female mice were randomly divided into two groups of 12, including PCOS group (P) and NC group (N). The PCOS group mice were subcutaneously injected with DHEA (6mg·100g-1·day-1) dissolved in the sterile soybean oil solution (0.2ml) for 20 consecutive days.The NC group were subcutaneously injected with sterile soybean oil (0.2ml) for 20 consecutive days.On the 21 day, the mice from each group were mated with fertile males of the same strain to induce pregnancy at five in the afternoon.On Day 4 of pregnancy, the mice were killed by the cervical dislocation to collect endometrial samples by squeezing. One sample set is mixed by the same endometrial samples of 3 mice.