Root of self-grafted pumpkin and grafted cucumber under salt stress

Tissue samples (root of pumpkin scion /pumpkin rootstock and cucumber scion/pumpkin rootstock) treated with NaCl for 24 h were ground into powder in a liquid nitrogen freezing state, and about 100 mg were taken out. RNA extraction was carried out using Tranzol kit (TransGen, Beijing), RNA dissolving solution was added to dissolve RNA into a 1.5 mL centrifuge tube, and then immediately frozen with liquid nitrogen. It was placed in a foam box with dry ice and sent to a transcriptome sequencing company for transcriptome sequencing (Vazyme, Beijing). Transcriptional construction and sequencing were completed in Vazyme Biotech Co., Ltd,.