Genome level identification of transcription start sites by nanoCAGE sequence in soybean (Glycine max).

Identification of the transcription start sites (TSS) of genes is critical for understanding promoter regions and transcription initiation. One well characterized and widely used method to determine TSSs is CAGE (Cap Analysis of Gene Expression). CAGE has been commonly used in animal studies, yet in plants species, the precise TSS and core promoter landscape is not sufficient yet, no experiment has been taken on soybean to identify TSS neither. Soybean is one economically valuable species used for both food supply and oil. In this study, we present the main results of nanoCAGE-seq to reveal the genome-wide TSS conducted on shoot and root in soybean Williams 82. Soybean (Wm 82) was grown under white light condition (300 µmol•m-2•s-1) with a 12 h light/12 h dark circadian rhythm for 2 weeks. Shoots and roots were collected separately and used for nanoCAGE library construction.