Loss of O-glycans in NiV G does not affect NiV F incorporation in pseudotyped virions, but affects NiV F processing and pseudotyped viral entry.

A) Western blot of NiV/VSV viral lysates. NiV G (top panel) and NiV F (bottom panel) were detected as described in Fig 2. B) F incorporation and F processing of the NiV mutant pseudotyped virions determined by densitometry. F incorporation values were normalized to those of wt NiV. F processing was measured by the ratio of F1/ (F0+F1) for NiV pseudotyped virions. N = 3. Statistically significant differences, as determined by a Student's t-test, are marked with an * (p<0.01). C) Viral entry of NiV/VSV virions at log dilutions of viral input. Values for the mutant virions are compared to wt NiV values. Pseudotyped virions expressing only NiV G (no NiV F) were used as a negative control. Averages and standard deviations are shown. N = 3.