Genome-wide transcriptional responses of Streptococcus gordonii Challis to AFYWWFY heptamer.

Transcriptome profiling of mid-log S. gordonii cells treated for 30 minutes with 1 µM of AFYWWFY heptamer dissolved in dimethyl sulfoxide (DMSO). One-condition experiment. S. gordonii were grown statically in 10 ml volumes to mid-log phase at 360C in 5% CO2 and then treated with either heptamer or solvent-only control and incubated for an additional 30 minutes before harvesting for RNA preparation. Experiment was repeated in different media: Todd Hewitt (TH; Difco) and chemically defined FMC medium (Terleckyj, B., Willett, N., P., and G. D. Shockman. 1975. Growth of Several Cariogenic Strains of Oral Streptococci in a Chemically Defined Medium. Infect Immun. 11(4):649–655). Biological replicates in TH: 1 control, 1 treated, 6 replicates per array. Biological replicates in defined medium: 2 control, 2 treated, 6 replicates per array. Dye swaps were performed for each sample set.