Single cell RNA sequencing of mouse male and female liver cells with and without acetaminophen-induced injury

We performed transcriptomic profiling of mouse whole liver cells perfused in situ with collagenase and then mechanically dissociated into single cell suspensions, to garner about 40% hepatocytes and 60% non-parenchymal cells. Cells were isolated from male and female C57BL/6J mice that received either PBS vehicle control, or 400 mg/kg acetaminophen (APAP) and perfused 48 hours after injury. Global transcriptomic profiling at single cell resolution of murine liver cells. 4 samples: 1) Male PBS vehicle control, 2) Female PBS vehicle control, 3) Male APAP-Injured, 4) Female APAP-Injured. Cells were isolated on two days, with 48 hours between, but sequenced all together on the same chip.