RNA-seq analysis of the effects of single and compound deletion of Moz (Kat6a) and/or Dlx5 during mouse craniofacial development

The purpose of these data is to investigate the genetic perturbations which underlie the cleft palate phenotype in Kat6a and Dlx5 mutant mouse embryos. The palate develops from the maxillary portion of the first pharyngeal arches, commencing between E10.5 and E11.5 of mouse gestation. The pharyngeal arches were taken from E10.5 female mouse embryos for each of the following genotypes: wild type (WT), Kat6a heterozygous knockout (KO), Dlx5 heterozygous KO, Kat6a and Dlx5 heterozygous KO, Dlx5 homozygous KO, Kat6a homozygous KO. There are four biological replicates for each genotype, giving a total of 24 samples. Batch effects, which were corrected for, arose from samples being derived from two different mouse facilities.本数据集旨在解析Kat6a与Dlx5基因突变小鼠胚胎腭裂表型背后的遗传扰动机制。腭起源于第一鳃弓的上颌突，其发育起始于小鼠妊娠阶段的胚胎第10.5天至第11.5天（E10.5至E11.5）。本研究采集了胚胎第10.5天的雌性小鼠胚胎的第一鳃弓样本，对应以下6种基因型：野生型（wild type, WT）、Kat6a杂合敲除型（Kat6a heterozygous knockout, KO）、Dlx5杂合敲除型（Dlx5 heterozygous KO）、Kat6a与Dlx5双基因杂合敲除型、Dlx5纯合敲除型以及Kat6a纯合敲除型。每种基因型设置4个生物学重复，总样本量为24份。样本来源于两家不同的小鼠饲养设施，由此产生的批次效应已完成校正。