Urine miRNA transcriptome of prostate cancer patients [Validation cohort]. Urine miRNA transcriptome of prostate cancer patients [Validation cohort]

Intra-individual stability of the urine miRNA transcriptome was examined by investigating longitudinal changes over time in a cohort of patients with localized prostate cancer. Using training and validation cohorts, urinary miRNA biomarkers are characterized and validated their utility to identify aggressive prostate cancer. Overall design: miRNAs are isolated from urinary cell sediments. Discovery cohort is composed of 22 samples from 10 patients and used to measure intra-individual stability. Training cohort that are composed of urine samples from 49 low-risk prostate cancer patients and 50 high-risk prostate cancer patients are used to identify urine biomarker to predict cancer aggressivity and generate a predictive model. The predictive model is validated using an independent validation cohort with 29 low-risk and 15 high-risk (based on NCCN-risk, 11 high-risk based on GS-risk) prostate cancer patients. Control samples are used for data normalization.