Transcriptomic sequencing after MALAT1 knockdown in A431 cells. Homo sapiens

Cutaneous squamous cell carcinoma (CSCC) is the most common metastatic skin cancer, and its incidence is increasing globally. In this study, metastasis-associated in lung adenocarcinoma transcript 1 (MALAT1), a long non-coding RNA, was identified to be significantly up-regulated in CSCC tumors and cell lines. We conducted RNA sequencing to characterize gene expression changes in MALAT1-silenced cutaneous squamous cell carcinoma cells (A431). Overall design: RNA-Seq analysis of CSCC cells followed by siRNA-induced gene knockdown of MALAT1. Total RNAs from negative control (NC ASO) and MALAT1 ASO-treated CSCC cells (antisense oligonucleotide) were extracted. The samples were sequenced using Illumina sequencing.