RNA-Seq of sorted E10.5 and E11.5 AGM cell populations from Gata3-GFP embryos

The aim of this study was to examine the whole transcriptome of pools of 20 cells of Gata3-expressing AGM subpopulations (haematopoietic [HC], mesenchymal [MC], sympathetic nervous system [SNS], and endothelial [EC]) and compare them to their non-Gata3 expressing counterparts Cells were sorted into: Gata3+ EC: GFP+ Cdh5+ CD41- CD45-, Gata3- EC: GFP- Cdh5+ CD41- CD45-, Gata3+ HC: GFP+ CD41+ CD45+, Gata3- HC: GFP- CD41+ CD45+, Gata3+ MC: GFP+ Pdgfrb+, Gata3- MC: GFP- Pdgfrb+, Gata3+ SNS: GFP+ Ngfr+ and Gata3- SNS: GFP- Ngfr+. Each population was then sorted into the lysis buffer in a 96-well plate at 20 cells per well.本研究旨在检测表达Gata3的主动脉-性腺-中肾区（aorta-gonad-mesonephros region, AGM）亚群各20个细胞混合样本的全转录组，这些亚群包括造血细胞（haematopoietic cell, HC）、间充质细胞（mesenchymal cell, MC）、交感神经系统细胞（sympathetic nervous system, SNS）以及内皮细胞（endothelial cell, EC），并将其与不表达Gata3的对应细胞群进行比较。细胞通过流式分选分为以下群：Gata3阳性内皮细胞（Gata3+ EC）：绿色荧光蛋白（green fluorescent protein, GFP）阳性、钙粘蛋白5（cadherin 5, Cdh5）阳性、CD41阴性、CD45阴性；Gata3阴性内皮细胞（Gata3- EC）：GFP阴性、Cdh5阳性、CD41阴性、CD45阴性；Gata3阳性造血细胞（Gata3+ HC）：GFP阳性、CD41阳性、CD45阳性；Gata3阴性造血细胞（Gata3- HC）：GFP阴性、CD41阳性、CD45阳性；Gata3阳性间充质细胞（Gata3+ MC）：GFP阳性、血小板衍生生长因子受体β（platelet-derived growth factor receptor beta, Pdgfrb）阳性；Gata3阴性间充质细胞（Gata3- MC）：GFP阴性、Pdgfrb阳性；Gata3阳性交感神经系统细胞（Gata3+ SNS）：GFP阳性、神经生长因子受体（nerve growth factor receptor, Ngfr）阳性；Gata3阴性交感神经系统细胞（Gata3- SNS）：GFP阴性、Ngfr阳性。随后将每个分选得到的细胞群以每孔20个细胞的密度接种至96孔板的裂解缓冲液中。