In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8+ T cell fate decisions [microarray_dataset1]

The role of nutrient signaling processes in the fate decision of CD8 is incompletely understood. By performing in vivo pooled CRISPR-Cas9 screening, we uncovered nutrient signaling processes underpinning the dynamics and heterogeneity of CD8 T cell fate decisions. We used microarray to compare gene expression of sgNTC (‘spike for sgPofut1’)- and sgPofut1-transduced P14 cells from the dual-color transfer system at day 5 and day 7.5 post-infection from spleen; We used microarray to compare the global transcription profiles of terminal effector cells (TE′, KLRG1hiCXCR3loCD127lo), memory precursor effector cells (MP, KLRG1loCXCR3hiCD127hi) and intermediate cells (TINT, CXCR3hiCD127lo) from sgNTC-transduced P14 cells at day 7.5 post-infection from spleen.