Atomic Structure of Amyloid Crystals

3D ED/MicroED data collected on micro-crystals of a fragment of amyloid-beta peptide. The specimen was prepared using the Preassis method (Zhao et. al. Nature Communications 2021) on Quantifoil R2/1 (300 mesh) grid. 3D ED/MicroED data were collected on a 200kV JEOL JEM-2100 LaB6 TEM equipped with a Timepix hybrid pixel detector. A Gatan 914 cryo-transfer holder was used to keep the specimen at liquid nitrogen temperature during data collection. The rotation speed of goniometer, exposure time, electron dose rate and camera length were 0.23 degree/s, 0.5 s, 0.1 e-/Å2/s and 30cm, respectively. The software Instamatic was used for electron diffraction data collection. The rotation range of individual dataset was limited between 40 degree and 50 degree due to beam damage.

The data was saved in TIFF, SMV, and DM3 (for REDp processing) format. Information of the individual data collection can be found in the cRED_log file. Input files for PETS processing and XDS processing (automatically generated) are also included.