Cut-and-Run in situ factor profiling maps DNA binding and 3D contact sites at high resolution

Unlike Chromatin Immunoprecipitation (ChIP), which fragments and solubilizes total chromatin, Cut-and-Run is performed in situ, allowing for both high-resolution chromatin mapping and probing of the local chromatin environment与需对总染色质进行片段化与溶解的染色质免疫沉淀（Chromatin Immunoprecipitation, ChIP）不同，切割释放法（Cut-and-Run）可在原位完成实验，能够同时实现高分辨率染色质图谱绘制以及局部染色质环境的探查。