Systematics of Lepidothrix manakins (Aves: Passeriformes: Pipridae) using RADcap markers

Although recent molecular phylogenetic analyses of Lepidothrix
manakins (family Pipridae) have helped clarify their evolutionary
relationships, the placement of several lineages remains in question
because of low or conflicting branch support. In particular, the
relationship of L. coronata to other members of the genus and
relationships within the L. nattereri+L. vilasboasi+L. iris clade have
been difficult to resolve. We used RADcap to collect restriction
site-associated DNA sequence data and estimate the first subspecies-level
phylogeny of the genus Lepidothrix (17 of 18 currently recognized
subspecies), and we included extensive geographic representation of the
widespread and phenotypically variable L. coronata. We found strong
support for the phylogenetic position and monophyly of L. coronata, and we
resolved two clades separated by the Andes that, along with previous
divergence time estimates and our assessment of morphological and vocal
evidence, suggest the presence of two biological species: Velvety Manakin
(L. velutina) west of the Andes and Blue-capped Manakin (L. coronata) east
of the Andes. Species-level relationships within the L. nattereri+L.
vilasboasi+L. iris clade remained poorly resolved in concatenated and
coalescent-based analyses, with SNAPP analyses suggesting that the lack of
reciprocal monophyly is due to extensive allele sharing among these taxa.
Finally, we confirmed a previously documented hybrid between L. coronata
and L. suavissima as an F1 individual, consistent with the view that
hybridization between these two species is a rare event and that
postmating reproductive barriers prevent successful backcrossing.