Transcriptional profiling of the adult mouse retina in wild-type and Nrl deficient mice

We performed mRNA transcriptional profiling of mouse retina in the wild-type and Nrl-null context to determine Nrl-dependent gene expression We sequenced cDNA libraries made from poly+ selected RNA from retinas of litter-matched WT and Nrl-/- adult mice at postnatal day 21 (P21) (WT vs Nrl-/-, n=5 and n= 6 resp.) Differential mRNA-seq testing of 21-day old wild type (WT) and Nrl-/- mice generated from polyA enriched RNA本研究对野生型（wild-type）与Nrl敲除（Nrl-null）背景的小鼠视网膜开展mRNA转录组分析，旨在明确依赖于Nrl的基因表达模式。我们对出生后第21天（P21）的同窝配对成年野生型（WT）及Nrl-/-小鼠的视网膜样本，提取经poly+选择（poly+ selected）富集的RNA后构建cDNA文库并开展mRNA测序差异表达分析；其中野生型组与Nrl敲除组的样本量分别为n=5与n=6。