File S1 - Molecular Evolutionary Analysis of the Alfin-Like Protein Family in Arabidopsis lyrata, Arabidopsis thaliana, and Thellungiella halophila

Figure S1 in File S1. Amino acid sequence alignment for 20 AL proteins by ML methods with bootstrapping analysis (1000 reiterations). The DUF3594 domain and PHD-finger are indicated by yellow and blue boxed letters. The positively selected codon sites are indicated by red arrows. The amino acids in red box display the altered key site as Lee et al [16]. Figure S2 in File S1. Phenotype response of 35S-AtAL7 A. thaliana lines to normal condition (left), 150 mM NaCl (middle), and 200 mM NaCl (right). Seedlings of vector (upper left), 35S-AtAL7-02 (upper right), 35S-AtAL7-04 (bottom left), and 35S-AtAL7-07 (bottom right) transgenic lines were germinated on a 1/2MS agar plate for 3 days, then transferred to another MS agar plate supplemented with 150 mM NaCl (middle) and 200 mM NaCl (right) for 5 days. Figure S3 in File S1. Diagram of AtAL3 and AtAL7 and their T-DNA insertion mutants. Figure S4 in File S1. Phenotype response of A. thaliana AL mutants to normal condition (left) and 150 mM NaCl (right). Seedlings of wild type (upper left), al7-1 (upper right), al7-2 (bottom left), and al3 (bottom right) mutants were germinated on a 1/2MS agar plate for 3 days, then transferred to another 1/2MS agar plate supplemented with 150 mM NaCl (right) and without (left) for 4 days. (DOC)