The mRNA-lncRNA-circRNA network profiling of Turner syndrome patient-derived induced pluripotent stem cells and their derived cardiomyocytes

Heart deformity is the leading cause of mortality in Turner syndrome (TS) patients. To investigate the dysregulated ceRNA network in cardiomyocytes (CMs) of TS patients, we employed a ceRNA microarray to profile the mRNA-lncRNA-circRNA network in induced pluripotent stem cells (iPSCs) and their derived CMs from healthy donors (WT) and TS patients. 3 WT and 3 TS patient-derived iPSC lines were generated from PBMCs. CMs were derived from these iPSC lines, respectively. The mRNA-lncRNA-circRNA networks in the WT and TS-derived iPSC and CM lines were examined with ceRNA microarrays that were capable of profiling 18k mRNAs, 68k lncRNAs and 88k circRNAs.