Rapid Genome-wide Recruitment of RNA Polymerase II Drives Transcription, Splicing and Translation Events during T Cell Responses

We wanted to verify optimal 4sU-labeling conditions and Ifng levels of activated Th0/Th1 cells.
Conclusion:
No apoptosis of 4sU-labeled cells for assigned concentrations and time points of activated Th0 cells. Numbers of Ifnγ producing Th1 cells increase until 4h but decrease later on. Each experiment was performed at least two times. BH3I-1 treatment was used to induce apoptosis (determined by Annexin V – PE), whereas heat shock (5min at 95°C) was used to induce cell death (determined by 7-AAD – PerCP). Unstained and single stained controls were included.