Gene expression profile at single cell level of cells from cholangiocarcinoma(CCA) and adjacent tissues.

Multiple tumor-associated cells in the complex CCA microenvironment.We used single cell RNA sequencing (scRNA-seq) to analyze the gene expression diversity of cells in the CCA and adjacent tissues. After dissociating tumor and adjacent tissues, lysing red blood cells and removing dead cells, candidate cells were scaptured to analyze used scRNA-seq.