Absence of IMC biogenesis does not interfere with formation of sub-pellicular microtubules.

(A) ddRab11BN125I parasites co-expressing the Golgi marker GRASP-RFP (red) were treated without and with Shld-1 for 24 h and labelled with the indicated antibodies (green). In presence of Shld-1 no IMC is formed, but nuclear and Golgi division appears normal (B) ddRab11BN125I parasites co-expressing IMC1-YFP (green) were grown in absence and presence of Shld-1 for 24 h and subsequently labelled with anti-MORN1 (red). In presence of inducer the centrocone close to the nucleus appears normal (arrow head). However, disruption of IMC formation inhibits formation and relocalisation of the posterior polar ring of daughter cells (white arrow). (C and D) ddRab11BN125I parasites co-expressing mCherry-α-Tubulin (red) were treated without and with inducer for 24 h and labelled with the anti-IMC1 antibody (green) or with anti-MORN1 antibody (green), respectively. Abrogation of IMC formation does not inhibit assembly and growth of the sub-pellicular microtubules (white arrow). Nevertheless, no formation of the basal complex can be observed despite presence of initial daughter formation (see inlet). Scale bar represents 5 µm.