Improved Reference Genome Sequence of <i>Onygena corvina</i>

We present an improved <i>O.</i> <i>corvina</i> genome assembly that was generated through a high-quality hybrid approach that combines Illumina short-read and Oxford Nanopore long-read sequencing. The new assembly consists of only 13 contigs totaling 21.8 Mb, with an N50 of 4.4 Mb, and has a completeness of 98.97%. A total of 7,232 protein-coding genes were identified using an integrative approach that combines de novo predictions, homology-based inferences, and RNA-sequencing–guided evidence.

本研究报道了一套优化后的*O. corvina*基因组组装结果，该组装通过结合Illumina短读长测序与牛津纳米孔（Oxford Nanopore）长读长测序的高质量混合测序策略构建而成。该新组装仅包含13个重叠群（contig），总长度达21.8 Mb，其N50值为4.4 Mb，基因组完整度达98.97%。本研究采用整合从头预测（de novo prediction）、同源推断以及RNA测序（RNA-sequencing）辅助证据的综合分析方法，共注释得到7232个蛋白质编码基因。

搜集汇总

背景与挑战

背景概述

该数据集提供了真菌Onygena corvina的改进参考基因组序列，通过混合测序方法获得高质量组装，包含13个contigs（总大小21.8 Mb，N50为4.4 Mb，完整度98.97%）和7,232个蛋白质编码基因的注释。数据集包含基因序列、蛋白质序列、GFF3注释和CAZymes分析等文件，适用于角蛋白降解和蛋白酶相关研究，支持工业微生物学应用。