RNA-sequencing of RNA extracted from WT and P2Y2 KO cardiac adipose-derived stem cells (cADSC), untreated or treated with UTP (100 µm)

Our goal was to identify UTP-regulated genes in WT cADSC and P2Y2 KO cADSC in the frame of the potential therapeutic transplantation of these cells into the mouse ischemic heart after a myocardial infarction. We identified UTP-regulated genes involved in inflammation and the immune response, UTP target genes linked to extracellular matrix organization such as matrix metallopeptidases and various collagen types, UTP-regulated genes related to macrophage chemotaxis and differentiation into pro-fibrotic foam cells and UTP target genes linked to angiogenesis regulation and the response to hypoxia. Overall design: 8 samples were analyzed; 2 different pools of RNA from 2 untreated WT cADSC cultures, 2 pools of RNA from 2 UTP-treated (100 µm) WT cADSC cultures, 2 pools of RNA from 2 untreated P2Y2 KO cADSC cultures and 2 pools of RNA from 2 UTP-treated (100 µm) P2Y2 KO cADSC cultures.