Gene signature of the embryonic meniscus

Analysis of gene expression in E16 mouse meniscus, articular cartilage, and cruciate ligaments. Limbs were dissected from E16 CD-1 mice. Samples were frozen in OCT and cryosectioned. Meniscus, articular cartilage, and cruciate ligament were isolated using laser capture microdissection. Total RNA was isolated from these tissues, amplified, and gene expression was analyzed using microarrays. Three biological replicates were analyzed for each tissue type. Total RNA extracted from E16 mouse meniscus, articular cartilage, and cruciate ligaments本数据集针对E16胎龄小鼠的半月板、关节软骨及十字韧带开展基因表达分析。具体实验流程为：从E16胎龄的CD-1小鼠体内分离四肢；将样本置于OCT包埋剂中冷冻并进行冰冻切片；通过激光捕获显微切割（Laser Capture Microdissection）分离得到半月板、关节软骨及十字韧带；从上述组织中提取总RNA，经扩增后利用基因芯片进行基因表达分析；每种组织类型设置三次生物学重复进行分析。提取自E16胎龄小鼠半月板、关节软骨及十字韧带的总RNA。