Protoarray-ATS_110525

ProtoArray® screening. The initial screening was performed using ProtoArray® Human Protein Microarrays v.4.0 (Thermo Fisher Scientific, Waltham, MA, USA), which are loaded with 9480 proteins, as described previously (1). A total of 20 serum samples (10 each from HDs and patients with atherosclerosis) were used to select antigenic proteins specifically recognized by serum immunoglobin G (IgG) antibodies. The results were analyzed using Prospector (Thermo Fisher Scientific) software based on M-statistics. When comparing the two groups, a positive cutoff value was calculated for each protein using M-statistics. The percentage of the samples in each group that showed an immune response above the cutoff value was determined and the P-value for the significance of the difference between the two groups was calculated, as previously described (2). Ref. 1. Hamanaka S, Nakagawa T, Hiwasa T, Ohta Y, Kasamatsu S, Ishigami H, Taida T, Okimoto K, Saito K, Maruoka D, et al.: Investigation of novel biomarkers for predicting the clinical course in patients with ulcerative colitis. J Gastroenterol Hepatol 33: 1975-1983, 2018. doi: 10.1111/jgh.14297. PMID:29869393 Ref. 2. Vermeulen N, de Béeck KO, Vermeire S, Van Steen K, Michiels G, Ballet V, Rutgeerts P and Bossuyt X: Identification of a novel autoantigen in inflammatory bowel disease by protein microarray. Inflamm Bowel Dis 17: 1291-1300, 2011. doi: 10.1002/ibd.21508. PMID:21560193