ChIP-seq identification of CrsL (MSMEG_5890) binding sites in Mycobacterium smegmatis

CsrL (MSMEG_5890) associates with CarD and RNA polymerase in mycobacteria. For ChIP-seq, we used Mycobacterium smegmatis strain with CrsL (LK3051), where an additional copy of CrsL under anhydrotetracycline inducible promoter was stably inserted into M. smegmatis genome. CrsL-FLAG was expressed in exponential phase and ChIP performed at mid-exponential phase of growth.