Proteinase-Activated Receptor 4 (PAR4) mediates cell membrane blebbing in a Gaq/11, Gai independent, RhoA and ß-arrestin-dependent manner.

To gain a comprehensive understanding of *-arrestin mediated signaling downstream of PAR4 and to guide future studies, we undertook RNAseq analysis of PAR4 activated genes in control cells and in cells lacking *-arrestin-1/-2. The results of this analysis revealed PAR4 regulation of genes involved in processes including blood coagulation and circulation, cell-cell adhesion, sensory perception and neuron-neuron synaptic transmission, terms that relates back to known function of PAR4. RNA profiles of beta-arrestin 1/2 double knockout cells treated with and without PAR4 agonist with respective controls. 2 replicates each.