Landscape of CD4+Foxp3+ Treg cells in the liver of neonatal mice

To study the heterogeneity of regulatory T cells (Tregs) in various organs, we performed single-cell RNA sequencing on liver and splenic Treg cells obtained from 12-day and 7-week old mice. Unique Treg subsets in neonatal liver were found and their impact on liver development and maturation was investigated. Overall design: To fully characterize liver Treg cells in neonatal mice, we performed scRNA-seq on sorted liver and splenic CD4+Foxp3GFP+ Tregs from 12-day-old Foxp3GFP-Cre Rosa26loxP-Stop-loxP-YFP mice. We also compared it with the scRNA-seq of Tregs purified from the liver and spleen of 7-week-old mice.