GWAS on salt stress responses in root system architecture parameters of wild tomato (S. pimpinellifolium)

The population of +/2 200 accessions of wild tomato was screened with the protocol described here with the only exception that the plants were transferred 4 days after germination (rather than 3 - described in the protocol). The images were analyzed using the SmartRoot for days 0, 1, 2, 3, and 4 after transfer to treatment plates (0 or 100 mM NaCl, 1/4 MS, 0.5% sucrose, 0.1% MES, 1% Dashin agar). The data analysis was performed as described here, while the pareto front calculations were done according to Chandrasekhar & Julkowska paper (preprint here). The GWAS was performed using the ASReml script similar to Awlia et al. (2021).