Significance of topological congruence between H. erato and H. melpomene phylogenies.

Phylogenies were estimated separately for the clades of H. erato and H. melpomene and in a combined analysis of the Heliconius. The significance of the cophylogeny mapping analyses was estimated based on minimising total reconstruction costs with Jane 3, either by randomising the leaf associations (column 2) or by randomizing the H. melpomene phylogeny (column 3). The significance of the pairwise distance correlation was calculated, at the root of the H. melpomene phylogeny (column 4) and at the root of the H. erato phylogeny (column 5), using TreeMap 3. Each p-value was estimated with 1000 Monte-Carlo replicates, and the 95% confidence upper bound was calculated for each using Wilson's score interval for binomial proportions [68].