Mechanism of activation of porcine dendritic cells by an alpha-D-glucan nanoparticle adjuvant and nanoparticle/poly(I:C) combination adjuvant.

RNA sequencing was performed to identify immune-related genes that were differentially expressed in porcine monocyte-derived dendritic cells following stimulation with a novel phytoglycogen-based nanoparticle (Nano-11) and the TLR3 agonist poly(I:C). Comparative gene ontology and KEGG pathway enrichment analyses were performed to identify differentially expressed genes in porcine DCs induced by Nano-11 and the combination adjuvant.