Single cell RNA seq of intestinal immune cells and PBMCs in salt-sensitive hypertension

We perfomred sc RNA seq of intetinal immun cell and PBMCs to figure out the roles of intestine in development of salt-sensitive hypertension. We fed a high salt diet(4% NaCl) or a normal salt diet(0.2% NaCl) to Dahl-salt senstive rats for 4 weeks. We isolated immune cells from small instesinal lamin propria and blood.本研究针对肠道免疫细胞（intestinal immune cells）与外周血单个核细胞（Peripheral Blood Mononuclear Cells, PBMCs）开展单细胞RNA测序（single-cell RNA sequencing, sc RNA-seq），以期阐明肠道在盐敏感性高血压发生发展中的作用。本研究向达尔盐敏感大鼠（Dahl salt-sensitive rats）饲喂含4% NaCl的高盐饮食或含0.2% NaCl的正常盐饮食，持续4周。随后从小肠固有层（lamina propria）与血液中分离免疫细胞。