Expression data from human primary monocyte derived immature dendritic cells (iDC)

During HIV-1 infection, iDCs contribute to mounting an immune response and to viral dissemination, however most of the studies addressing iDCs-HIV-1interactions have been performed in standard 2D suspension cultures which do not reflect the 3D architecture of tissues in vivo. To overcome this limitation and to understand the role that the 3D environemt has we studied iDC biology as well as iDC-HIV-1 functional interactions in 3D collagen matrix comparing 3D collagen cultures to standard 2D suspension cultures. We used microarray data to analyze how the 3D environment affects the expression profile of monocyte derived iDCs under basal condition, LPS stimulation and in response to HIV-1 infection iDCs were either stimulated with LPS as positive control, infected with HIV-1 or left untreated and cultured in 2D suspension or 3D collagen for two days. Two days after RNA was exctracted and transcriptomes were determined by Clariom™ S Pico assay