A newly-identified IncY plasmids from multi-drug resistant Escherichia coli isolated from dairy cattle feces in Poland

Comprehensive whole-genome sequencing was performed on two multi-drug resistant Escherichia coli strains isolated from cattle manure from a typical dairy farm in Poland. The identified strains are resistant to beta-lactams, aminoglycosides, tetracyclines, trimethoprim/sulfamethoxazole, and fluoroquinolones. The complete sequences of the harbored plasmids revealed antibiotic-resistance genes (ARGs) located within many mobile genetic elements (e.g., insertional sequences or transposons), and genes facilitating conjugal transfer or promoting horizontal gene transfer. These plasmids are hitherto undescribed. Similar plasmids have been identified, but not in Poland. While similar plasmids (99% identity) have been found in environmental and clinical samples, none have been identified in farm animals.