MiSeq analysis on the RT-PCR amplicon of HCV from human sera.. Hepacivirus hominis

The study focused on the question of whether HCV/HIV coinfected hemophiliacs, who are suspected of being repeatedly exposed to unheated blood-related products, differ in the composition of hepatitis C viral quasispecies (genotypes and resistance mutations), from HCV monoinfected non-hemophiliacs with or without a history of whole blood transfusion. Twenty one clinical serum samples were included in this study for the analysis on the HCV core and NS3 protease region for genotyping and the NS3 protease region for naturally occurring resistance-associated variants, by Illumina MiSeq deep sequencing .