A new species of Pseudosperma and first record of P. aureocitrinum (Agaricales, Inocybaceae) from Pakistan

During the fungal exploration from the Himalayan moist temperate forests of Pakistan, different fruiting bodies of the genus Pseudosperma were collected that resulting in one new species, i.e., Pseudosperma khanspuricum, and one new record, Pseudosperma aureocitrinum. Pseudosperma khanspuricum is characterized by its campanulate, fibrillose, smaller (up to 2.2 cm), brownish yellow pileus with acute umbo, larger (up to 5.2 cm) stipe, creamy white to light yellow lamellae, and smaller (8.9 × 5.8 µm) basidiospores. The second species Pseudosperma aureocitrinum has been reported for the first time from Pakistan. Molecular phylogenetic evaluation deduced from the nrDNA internal transcribed spacer region (ITS) and larger subunit region (LSU) validates this as a new species and a new record, with this addition species of Psudosperma count to be 13 from Pakistan., , , # A new species of *Pseudosperma* and first record of *P. aureocitrinum* (Agaricales, Inocybaceae) from Pakistan

Dataset DOI: [10.5061/dryad.jq2bvq8mb](10.5061/dryad.jq2bvq8mb)

## Description of the data and file structure

The 2 fasta files (ITS, LSU) are the files used for phylogenetic analyses of Pseudosperma spp.

#### File: ITS.fas

Phylogenetic analysis of *Pseudosperma khanspuricum* (PQ637574, PQ654720, PQ654718)  and *Pseudosperma aureocitrinum* (PQ721832) based on ITS sequences using the Maximum Likelihood method.

#### File: LSU.fas

Phylogenetic analysis of *Pseudosperma khanspuricum* (PQ721844) based on LSU sequences using the Maximum Likelihood method.

These fasta files can be opened in BioEdit or any other phylogenetic software.,