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RNA Sequencing analysis of transcriptomics to investigate the bile stress response in L. paracasei L9

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NIAID Data Ecosystem2026-04-25 收录
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Purpose: To understand the bile salts resistance mechanisms in L. paracasei L9 Methods: Samples from L9 cultured with or without bile salts were sequenced on an Illumina Hiseq platform. Three independent biological replicates were produced including 6 samples in total. Results: Raw data were firstly processed through in-house perl scripts to generate clean data, and then clean date were mapped to the reference genome, getting about 8-10 million total mapped reads per sample. Overall design: L. paracasei L9 was cultured in MRS medium with 0.13% oxgall,f

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2020-09-29
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