Ileal gene expression in Duoxa-/- mice and cohoused littermate controls

Dual oxidases play a role in innate host defense at barrier epithelia. We examined the effect of loss of dual oxidase function (duoxa-/-) on gene expression in the mouse terminal ileum at homeostasis. To control for cage/litter effects, duoxa-/- were cohoused with wild type littermate controls. Ileal mucosal samples were obtained from duoxa-/- mice and cohoused wild type littermates from three separate cages (litters). For each cage, RNA was pooled from n=2-3 animals per genotype.双氧化酶（Dual oxidases）在屏障上皮的宿主先天免疫防御中发挥功能。我们考察了双氧化酶功能缺失（duoxa-/-）对稳态下小鼠回肠末端基因表达的影响。为控制笼舍效应与同窝效应，将duoxa-/-小鼠与野生型同窝对照小鼠共同饲养。从3个独立笼舍（同窝群）的duoxa-/-小鼠及其共同饲养的野生型同窝对照小鼠中获取回肠黏膜样本。针对每个笼舍，每个基因型取2~3只小鼠的RNA进行混合。