Transcriptome analysis of the zebrafish embryonic host response to Edwardsiella tarda infection using a static immersion systems [experiment A]. Danio rerio

In this study we analyzed the zebrafish embryonic host response induced by E. tarda (FL6-60) immersion. The E. tarda induced transcriptome profile was compared to those induced by either E. coli or Pseudomonas aeruginosa immersion using the same experimental setup. Overall design: All infection experiments were performed using mixed egg clutches of Albino strain zebrafish. At 24 hpf embryos were dechorionated using 2mg/ml pronase and left to recover for one hour in egg water. Subsequently embryos were immersed in a bacterial suspension (E. tarda (1E8 CFU/ml), E. coli (1E8 CFU/ml), Pseudomonas aeruginosa PAO1 (1E9 CFU/ml) or P. aeruginosa PA14 (1E9 CFU/ml)) and incubated for 5 hours at 28 ˚C. After the incubation period the embryos were snap-frozen in liquid nitrogen. All treatment groups were analyzed using a common reference approach.