The modulation of acetic acid pathway genes in Arabidopsis produces drought-stress tolerant plants

Gene expression profiles of control and drought-stressed shoots of WT and pTSPO-PDC1-5 was performed using the custom microarray Agilent-034592 WT and transgenic seeds were sown in trays containing Dio propagation mix no. 2 professional soil (Dio Chemicals, Japan) and germinated and grown at 22°C under a 16 h light/8 h dark cycle, and a 60 µmol m−2 s−1 photon flux density. Two weeks after germination, plants were subjected to drought treatment by removing excess water from the trays and ceasing any subsequent watering. Shoot samples were collected 11 days after drought stress treatment. Plants not subjected to drought stress treatment were used as control. Samples were immediately frozen in liquid nitrogen and stored at -80 degrees until RNA extraction.