VcdRP target identification

In this study we determined the target spectrum of the Vibrio cholerae dual RNA regulator VcdRP via pulse-expression of different variants followed by RNA-sequencing. V. cholerae ΔvcdRP cells carrying inducible pBAD expression vectors were grown to early exponential phase (OD600=0.1). Expression was induced by L-arabinose and total RNA samples were collected 15 minutes after induction. The experiment was performed with three independent biological replicates. The following vectors were used: pBAD1K-Ctrl (empty control plasmid), pBAD1K-VcdRP (with the full VcdRP sequence), pBAD1K-VcdR (with a stop codon at the third position of the VcdP peptide) or pBAD1K-VcdP (with a modified nucleotide sequence coding for the VcdP peptide alone).