Transcription profiling of eyes from wild type and Bma knock-out mice after different lengths of time in a 12:12-hr LD cycle or in constant darkness to identify daily rhythms in ocular gene expression

To explore daily rhythms of ocular gene expression in adult mice we performed the following experiments: i) Mice were entrained to a 12:12-hr light-dark (LD) cycle for 3 weeks. Then mice were transferred to constant darkness (DD) or remained in LD and eyes were collected at four-hour intervals over a three-day period; ii) Bmal1-/- mice and wild-type littermates were entrained to a 12:12-hr LD cycle for 3 weeks and eyes were collected at four-hour intervals over a one-day period in LD.为探究成年小鼠眼部基因表达的每日节律，我们开展了如下实验：i) 将小鼠置于12:12小时明暗光照周期（light-dark, LD）中适应性饲养3周，随后将其分为两组，一组转移至持续黑暗（constant darkness, DD）环境，另一组继续维持LD光照条件；在为期3天的实验周期内，每4小时采集一次眼部组织样本；ii) 将Bmal1-/-小鼠及其野生型同窝仔鼠置于12:12小时LD光照周期中适应性饲养3周，在LD环境下的为期1天的实验周期内，每4小时采集一次眼部组织样本。