Using Genomic Signatures to Elucidate Individual and Mixture Effects of Inorganic Arsenic and Manganese in Placental Trophoblasts. Using Genomic Signatures to Elucidate Individual and Mixture Effects of Inorganic Arsenic and Manganese in Placental Trophoblasts

HTR-8/SVneo human placental trophoblast cells were exposed to low-level environmentally relevant concentrations of iAs, Mn, and iAs-Mn mixtures. A microarray assay was performed to understand the changes and differences in gene expression in relation to single-metal and metal-mixtures Overall design: Placental trophoblasts were seeded and exposed to iAs (0.3µM, 1µM, 1.5µM), Mn (5µM, 10µM, 20µM), or an iAs-Mn mixture (0.3µM iAs+5µM Mn, 1µM iAs + 10µM Mn, 1.5µM iAs + 20µM Mn). Samples were developed in triplicate, and unexposed controlls were included as well. Cells were exposed for 24 hrs prior to RNA extraction.