Multi-Scale Integrated Cell (MuSIC) v1

Two central approaches for mapping cellular structure – protein fluorescent imaging and protein biophysical association – each generate extensive datasets but of distinct qualities and resolutions that are typically treated separately. The MuSIC map is designed to address this challenge, by integrating immunofluorescent images in the Human Protein Atlas with ongoing affinity purification experiments from the BioPlex resource. The result is a unified hierarchical map of eukaryotic cell architecture. In the MuSIC hierarchy, nodes represent systems and arrows indicate containment of the lower system by the upper. Node color indicates known (gold) or putative novel (purple) systems. The size of each circle is based on the number of proteins in the system. The relative height of each system in the layout is determined based on the predicted diameter of the system in MuSIC.