Transcriptome analysis revealed key genes and pathways of nitrate in the prevention of hypoxia-induced small intestinal epithelial injury

Our study helps to understand the transcriptome level changes in small intestines exposed to hypoxia after nitrate supplementation, to find out which genes or signaling pathways nitrate plays a role in by activating or inhibiting them. Overall design: In this study, we isolated small intestine from wild mice exposed to hypoxia (7%O2) for 7 days(Hypoxia) and gave them 4mM nitrate supplementation(Hypoxia+Nitrate). The Hypoxia group and the Hypoxia+Nitrate group (4 samples each) were subjected to RNA sequencing.