Target genes, interacting proteins, ubiquitination sites, and primers for OsWRI1a functional analysis

Nitrogen (N) deficiency-induced increases in the root-to-shoot biomass ratio in plants are adaptive in nature but suboptimal for agriculture. Understanding the regulatory mechanisms governing this developmental plasticity could help improve crop performance while reducing fertilizer application. We identified OsWRI1a (WRINKLED1a) as a regulatory hub coordinating rice root and shoot growth in response to external N supply, thereby stabilizing the root-to-shoot ratio. In roots, OsWRI1a enhances N-responsive development by promoting auxin accumulation. Meanwhile, shoot OsWRI1a stimulates tiller development and therefore shoot growth. We identified an elite OsWRI1a haplotype that minimizes root-to-shoot ratio fluctuation under N deficiency, improving N-use efficiency (NUE) and grain yield. Our findings reveal a central mechanism coordinating N-responsive growth allocation for sustainable agriculture., , , # Target genes, interacting proteins, ubiquitination sites, and primers for OsWRI1a functional analysis

Dataset DOI: [10.5061/dryad.z08kprrts](https://doi.org/10.5061/dryad.z08kprrts)

## Description of the data and file structure

Data S1. Common OsWRI1a target genes identified from the CUT&Tag and RNA-seq analyses. 

Target genes were definedfrom CUT&Tag as those associated with significant binding peaks (*P*-value < 0.01, fold change > 1.5) found in the genomic region encompassing the entire gene and extending 4 kb upstream and downstream. The criteria for defining differentially expressed genes (DEGs) down-regulated in *oswri1a* are fold change > 2 and *P*-value < 0.05 in RNA-seq analysis.

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Data S2. SNPs and Indels in the promoters of HJX74 *OsWRI1a* and *OsWRI1b*.

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Data S3. OsWRI1a-interacting proteins. 

OsWRI1a-FLAG protein and its interacting proteins were captured by incubating extracted proteins from *pAct::*O*sWRI1a-Flag* transgenic lines using magnetic beads...